It has, however, become apparent, that this simple approach is not sufficient for a detailed analysis of molecular alterations, which might be restricted to a specific tissue phenotype (e.g., tumor or healthy tissue, stromal or epithelial cells). The use of bulk tissue, which is not precisely characterized in terms of histology, has long been the basis for molecular analysis. This is a general problem of non-in situ techniques, whereas results from in situ techniques are often difficult to quantitate. Located in E&R Room 3107.The new opportunities of modern assays of molecular biology can only be exploited fully if the results can be accurately correlated to the tissue phenotype under investigation. The entire process can be monitored and documented, with images in the archiving workstation. The PixCell II transfers cells from paraffin-embedded and frozen tissue samples, as well as stained and immunolabeled slides. LCM preserves the exact morphologies of both the captured cells as well as the surrounding tissue. Single cells or large groups of cells can be captured, using a simple aim-and-shoot method, and processed for subsequent molecular analyses. The PixCell II performs Laser Capture Microdissection (LCM) using visible or fluorescent imaging. (313) 916-4220 Arcturus PixCell ® II Leica DM5500 B Laser Capture Microdissector The monthly schedule can be found on the facility door at E&R Room 3031. The system is located on the third floor of the E&R building at the main campus. The facility manager is available for basic training and troubleshooting. The facility operates on a scheduled basis, Monday through Friday from 8:00 a.m.

The system has its own acquisition and analysis software, LaserSharp 2000. The inverted microscope is equipped with an objective lens of 10X, 0.3 numerical aperture (NA), 40X, 1.30 NA, 63X, 1.40 NA, and 100X, 1.3 NA. The confocal microscope core facility at Henry Ford Hospital has a Bio-Rad MRC 1024 3-channel laser-scanning confocal imaging system, utilizing a krypton argon laser with 488, 568, and 647 nm wavelengths, mounted onto a Zeiss 100 Axiovert inverted microscope.

Arcturus pixcell ii manual